Tel/fax: +44 (0) 1904 561592

LPS Extraction Kit

Catalog Number: alm694763  Pack size: 100 Rxn.
€234.8
Цена может отличаться от указанной на сайте, актуальные цены уточняйте у менеджеров по email
CompositionLPS Extraction Kit 100 Preps Lysis Buffer 100 ml Purification Buffer 80 ml
Estimated delivery time1 week
DescriptionLipopolysaccharides(LPS)isamajorconstituentoftheoutermembraneof gram-negativebacteria.Someofitsfunctionsincludearoleintheresistance tophagocytosis,resistancetoserum,outermembranepermeabilitybarrier andasareceptorforadsorptionofsomebacteriophages.Thehotphenol- waterextractionmethodisusuallyusedforextractionofLPSbuttakeslong timeandthoseprocedureiscomplicated.Andthemethodhasmajor limitationsinourhandswhenattemptingtomanipulatesuchsmallquantities of cells. LPSExtractionKitisdesignedforrapid,convenientmicroscaleextractionof LPSfrombacterialcellsandthatisbroadlyapplicableamongdifferentgram- negative bacteria and appropriate for the small numbers of cells. Step 1. Lysis Thebacterialcellsarelysedbyorganicsolution.Phospholipidandprotein componentsofcellmembranedisruptedandcellcomponentsarereleasedin solution. Step 2. LPS purification PurificationofLPSamongthereleasedcellcomponentswithhighsalt concentration solution. Step 3. Washing and Elution Salts are briefly removed by a washing step for high quality LPS.
CHARACTERISTICS: • Broadly applicable among different gram-negative bacteria • Takes only 60minutes to extract LPS • Gives reproducibly high yields of LPS
Application NotesLipoplysaccharides Prep. Type: Solution.
StorageStore at 4℃ , and then stable for at least one year.
Shipment Conditions2 -8 ºC
Available for saleRussia, Ukraine, Belarus, Kazakhstan
   will be soon